Endogenous Antibody Interferences in Immunoassays
نویسندگان
چکیده
منابع مشابه
Interferences in Immunoassays
Interference in immunoassays is a serious but underestimated problem (Ismail et al, 2002a). Interference is defined as “the effect of a substance present in the sample that alters the correct value of the result, usually expressed as concentration or activity, for an analyte” (Kroll & Elin, 1994). Immunoassays are analytically sensitive and measurements can frequently performed without prior ex...
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Endogenous, circulating digoxin-like immunoreactive factors (DLIF) are known to cross react with many antisera used in digoxin assays, complicating the quantification of serum digoxin. We have explored ways to decrease or remove the interference from DLIF in such assays. Prolonging the assay incubation from 30 to 60 min decreased apparent digoxin concentrations (attributable to DLIF) by an aver...
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Urine drug screen (UDS) immunoassays are a quick and inexpensive method for determining the presence of drugs of abuse. Many cross-reactivities exist with other analytes, potentially causing a false-positive result in an initial drug screen. Knowledge of these potential interferents is important in determining a course of action for patient care. We present an inclusive review of analytes causi...
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ژورنال
عنوان ژورنال: Laboratory Medicine
سال: 2013
ISSN: 0007-5027,1943-7730
DOI: 10.1309/lmmurcfqhksb5yec